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Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules.

Markmiller S, Soltanieh S, Server KL, Mak R, Jin W, Fang MY, Luo EC, Krach F, Yang D, Sen A, Fulzele A, Wozniak JM, Gonzalez DJ, Kankel MW, Gao FB, Bennett EJ, Lecuyer E, Yeo GW
Cell. 2018 Jan 25;172(3):590-604.e13. doi: 10.1016/j.cell.2017.12.032. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
105284HR_G3BP1-V5-APEX2-GFPhomologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus
105285hPGK_V5-APEX2-GFPdrives expression of V5-NES-APEX2-GFP from human PGK promotor
105286pLIX403_UBAP2L_mCherrypuro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein
105287pLIX403_deltaUBA_UBAP2L_mCherrypuro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein lacking the UBA domain of UBAP2L

Antibodies from Article