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Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks.

Paix A, Folkmann A, Goldman DH, Kulaga H, Grzelak MJ, Rasoloson D, Paidemarry S, Green R, Reed RR, Seydoux G
Proc Natl Acad Sci U S A. 2017 Nov 28. pii: 1711979114. doi: 10.1073/pnas.1711979114. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
105242pAP1698-4mammalian eGFP
105243pAP2050-2mammalian GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker
105244pAP2042-1mammalian eGFP with TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker
105245pAP1716-1human eGFP with homology arms for Lamin A/C
105246pAP1791-1human eGFP with homology arms for RAB11A (recoded )
105247pAP1892-1human GFP11 with tagRFP 33bp downstream in Lamin A/C
105248pAP1893-1human GFP11 with tagRFP 33bp downstream in Lamin A/C (recoded)
105249sgPYM1human PYM1 sgRNA
105250pBS-AC3CtermGenomic-mCherrymouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3

Antibodies from Article