A high-conductance chemo-optogenetic system based on the vertebrate channel Trpa1b.
Lam PY, Mendu SK, Mills RW, Zheng B, Padilla H, Milan DJ, Desai BN, Peterson RT
Sci Rep. 2017 Sep 19;7(1):11839. doi: 10.1038/s41598-017-11791-z.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 106291 | pME-Trpa1b-2A-mCherry | Gateway middle entry construct containing zebrafish Trpa1b, 2A self-cleaving peptide and mCherry |
| 106403 | pCMV-Trpa1b-FLAG | Mammalian expression vector containing zebrafish Trpa1b with C-terminal FLAG tag. |
| 106405 | Tol2-cmlc2-Trpa1b-2A-EGFP | For expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes. |
| 106425 | Tol2-ngn1-Trpa1b-2A-mCherry | For expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons. |