A simple and versatile system for the ATP-dependent assembly of chromatin.
Khuong MT, Fei J, Cruz-Becerra G, Kadonaga JT
J Biol Chem. 2017 Nov 24;292(47):19478-19490. doi: 10.1074/jbc.M117.815365. Epub 2017 Oct 5.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 105498 | pET21-His6dNLP | Expression of N-terminally 6His tagged Drosophila nucleoplasmin-like protein |
| 105499 | pET24-ISWI-iCBD | Expression of Drosophila ISWI C-terminally tagged with Combined Sce VMA intein and chitin binding domain (iCBD) |
| 105500 | pET11a-dH2A | Expression of Drosophila histone H2A |
| 105501 | pET11a-dH2B | Expression of Drosophila histone H2B. An additional Ile codon was inserted after the initiating Met codon to enhance the protein expression level |
| 105502 | pET11a-dH3-dH4 | Co-expression of Drosophila histones H3 and H4 |
| 105503 | pET-NDH6 | Expression of an N-terminally truncated form of Drosophila topoisomerase I with a C-terminal 6His tag |