Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer Immunotherapy.
Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, Wehrspaun C, Tabach Y, Sharp PA, Lu TK
Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 105171 | p119 (FUGW-UbCp-rtTA3) | ubiquitin C promoter drives rtTA3 expression |
| 105172 | pLN342 (FuGW-SSX1p-mKate2) | SSX1 promoter drives mKate2 expression |
| 105173 | pLN343 (FuGW-H2A1p-mKate2 ) | H2A1 promoter drives mKate2 expression |
| 105174 | pLN351 (FuGW-S(YBX1)p-mKate2 ) | synthetic promoter YBX1 drives mKate2 expression |
| 105175 | pLN352 (FuGW-S(Elk1)p-mKate2) | synthetic promoter Elk1 drives mKate2 experssion |
| 105176 | pLN377 (FuGW-S(SP1)p-mKate2 ) | synthetic promoter SP1 drives mKate2 expression |
| 105177 | pLN379 (FuGW-S(E2F1)p-mKate2) | synthetic promoter E2F1 drives mKate2 expression |
| 105178 | pLN380 (FuGW-S(CEBPB)p-mKate2) | synthetic promoter CEBPB drives mKate2 expression |
| 105179 | pLN381 (FuGW-S(NFY)p-mKate2) | synthetic promoter NFY drives mKate2 expression |
| 105180 | pLN382 (FuGW-S(HIF1A)p-mKate2 ) | synthetic promoter HIF1A drives mKate2 expression |
| 105181 | pLN397 (FuGW-S(E2F1)p-Sponge7) | Module 2 - synthetic promoter E2F1 drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information) |
| 105182 | pLN410 (FuGW-S(E2F1)p-ECFP sponge control) | Module 2 control - synthetic promoter E2F1 drives ECFP only (no miR1 binding sites) |
| 105183 | pLN426 (FuGW-G5p-mKate2) | Module 3 - GAL4 synthetic promoter (with five binding sites) drives mKate2 expression |
| 105184 | pLN428 (FuGW-G5p-mKate2-1Pe ) | Module 3 - GAL4 synthetic promoter (with five binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe) |
| 105185 | pLN429 (FuGW-G8p-mKate2) | Module 3 - GAL4 synthetic promoter ( with eight binding sites) drives mKate2 expression |
| 105186 | pLN431 (FuGW-G8p-mKate2-1Pe ) | Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe) |
| 105187 | pLN432 (FuGW-G14p-mKate2) | Module 3 - GAL4 synthetic promoter( with fourteen binding sites) drives mKate2 |
| 105188 | pLN434 (FuGW-G14p-mKate2-1Pe ) | Module 3 - GAL4 synthetic promoter (with fourteen binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe) |
| 105189 | pLN466 (FuGW-S(cMYC)p-mKate2) | synthetic promoter cMYC drives mKate2 expression |
| 105190 | pLN472 (FuGW-G8p-STE-1Pe) | Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of STE transcripts containing miR1-Bs(Pe) |
| 105191 | pLN481 (FuGW-G8p-IL12-1Pe) | Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 transcripts containing miR1-Bs(Pe) |
| 105192 | pLN484 (FuGW-S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe) | Module 1 - synthetic promoter cMYC drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information) |
| 105193 | pLN491 (FuGW-G8p-CCL21-1Pe) | Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of CCL21 transcripts containing miR1-Bs(Pe) |
| 105194 | pLN492 (FuGW-G8p-antiPD1(HC)-1Pe) | Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab heavy chain transcripts containing miR1-Bs(Pe) |
| 105195 | pLN493 (FuGW-G8p-antiPD1(LC)-1Pe) | Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab light chain transcripts containing miR1-Bs(Pe) |
| 105200 | pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe) | Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe) |
| 105201 | pLN540 (FuGW-G8p-IL12-P2A-CCL21-1Pe) | Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 and CCL21 transcripts containing miR1-Bs(Pe) |
| 105202 | pLN552 (FuGW-S(USF1)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe) | Module 1 - synthetic promoter USF1 drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information) |
| 105203 | pLN553 (FuGW-S(MAFK)p-Sponge7) | Module 2 - synthetic promoter MAFK drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information) |
| 105204 | pLN592 (FuGW-S(USF1)p-mKate2) | synthetic promoter USF1 drives mKate2 expression |
| 105205 | pLN593 (FuGW-S(MAFK)p-mKate2) | synthetic promoter MAFK drives mKate2 expression |
| 105206 | pLN599 (pFuGW-hUbCp-hSTE1) | ubiquitin C promoter drives STE expression |
| 105207 | pLN520 (SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe)) | Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 1 intron - see PMID: 29056342 for detailed information) |
| 105209 | pLN189 (SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe)) | Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 2 intron - see PMID: 29056342 for detailed information) |
| 105210 | pLN193 (SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe)) | Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 3 intron - see PMID: 29056342 for detailed information) |