Multiplexed CRISPR/Cas9 Genome Editing and Gene Regulation using Csy4 in Saccharomyces cerevisiae.

Ferreira R, Skrekas C, Nielsen J, David F
ACS Synth Biol. 2017 Nov 21. doi: 10.1021/acssynbio.7b00259. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
103139	p413_Csy4NLS	Expresses Csy4-NLS under TEF promoter
103140	dCas9v2	TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site
103141	pCRA03	Triple gRNA expression separated with 28nt Csy4 motif