Single-Molecule Quantification of Translation-Dependent Association of mRNAs with the Endoplasmic Reticulum.
Voigt F, Zhang H, Cui XA, Triebold D, Liu AX, Eglinger J, Lee ES, Chao JA, Palazzo AF
Cell Rep. 2017 Dec 26;21(13):3740-3753. doi: 10.1016/j.celrep.2017.12.008.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
104982 | phage UbiC SNAP-KDEL | Lentiviral vector expressing SNAP-KDEL for ER labeling |
104984 | pSF4 TetCMV intron 4xGCN4 Renilla FKBP STOP 24xMS2v5 SV40 CTE polyA | Expresses ST-Renilla reporter in mammalian cells |
104998 | phage UbiC scAB-GFP | Lentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labeling |
104999 | phage UbiC NLS HA stdMCP stdHalo | Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling |
105000 | pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA | Expresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats |
107230 | pUC57-PP7v3 | Expresses 24x optimized PP7 stem loops (version 3) in bacterial cells |