Evolving accelerated amidation by SpyTag/SpyCatcher to analyze membrane dynamics.
Keeble AH, Banerjee A, Reddington SC, Ferla MP, Howarth M, Khairil Anuar INA
Angew Chem Int Ed Engl. 2017 Oct 10. doi: 10.1002/anie.201707623.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 102827 | pDEST14-SpyCatcher002 | Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins |
| 102828 | pDEST14-Avitag-SpyCatcher002 | Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation |
| 102829 | pDEST14-Cys-SpyCatcher002 | Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents |
| 102830 | pDEST14-SpyCatcher002 EQ | Bacterial expression of unreactive version of SpyCatcher002 |
| 102831 | pET28a-SpyTag002-MBP | Bacterial expression of SpyTag002 fused to maltose binding protein |
| 102832 | pET28a-SpyTag002 DA-MBP | Bacterial expression of unreactive version of SpyTag002 fused to maltose binding protein |