Split-ubiquitin system for identifying protein-protein interactions in membrane and full-length proteins.
Grefen C, Lalonde S, Obrdlik P
Curr Protoc Neurosci. 2007 Oct;Chapter 5:Unit 5.27. doi: 10.1002/0471142301.ns0527s41.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
105081 | pMetYC-Dest | Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon. |
105083 | pNX32-Dest | Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning |
105085 | pXN22-Dest | Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning |
105086 | pXN21-Dest | Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning |