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Split-ubiquitin system for identifying protein-protein interactions in membrane and full-length proteins.

Grefen C, Lalonde S, Obrdlik P
Curr Protoc Neurosci. 2007 Oct;Chapter 5:Unit 5.27. doi: 10.1002/0471142301.ns0527s41. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
105081pMetYC-DestYeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.
105083pNX32-DestYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning
105085pXN22-DestYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning
105086pXN21-DestYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Antibodies from Article