CRISPR/Cas9 and TALENs generate heritable mutations for genes involved in small RNA processing of Glycine max and Medicago truncatula.
Curtin SJ, Xiong Y, Michno JM, Campbell BW, Stec AO, Cermak T, Starker C, Voytas DF, Eamens AL, Stupar RM
Plant Biotechnol J. 2017 Oct 31. doi: 10.1111/pbi.12857.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 104779 | pSC5 | pSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary vector |
| 104780 | pSC6 | pSC218UG-At Ubiquitin10:Gateway expression cassette binary vector |
| 104784 | pSC10 | pAH595-gRNA 2xplex entry cassette |
| 104786 | pSC12 | pNB184-Cas9 entry cassette |
| 104803 | pSC29 | CRISPR/Cas9 2xplex gRNA targeting Medtr4g094545 (Hen1). Also expresses Cas9 from Gmubi promoter. |
| 104817 | pSC43 | CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from Gmubi promoter from rolD promoter |
| 104818 | pSC44 | CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from AtUBQ10 promoter. |
| 104819 | pSC45 | CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-2). Also expresses Cas9 from Gmubi promoter. |
| 104824 | pSC50 | CRISPR/Cas9 2xplex gRNA targeting Glyma.08g081600, Glyma.05g126600 (Hen1ab). Also expresses Cas9 from Gmubi promoter. |
| 104825 | pSC51 | CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoter |