Anion-conducting channelrhodopsins with tuned spectra and modified kinetics engineered for optogenetic manipulation of behavior.

Wietek J, Rodriguez-Rozada S, Tutas J, Tenedini F, Grimm C, Oertner TG, Soba P, Hegemann P, Wiegert JS
Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.1038/s41598-017-14330-y. PubMed

Plasmids from Article

ID	Plasmid	Purpose
98165	p-mCherry-C1-iC++	artificial anion conducting channelrhodopsin (aACR). Activation max 488 nm; off-kinetics 35 ms. Codon optimized for mammalian expression.
98166	p-mCherry-C1-Phobos	blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.
98167	p-mCherry-C1-Aurora	red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.
98168	p-mCherry-C1-iC++_CA	slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with cyan light, inactivation max with 590 nm (accelarates closure to ms). Codon optimized for mammalian expression.
98169	p-mCherry-C1-Phobos_CA	slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.
98170	p-mCherry-C1-Aurora_CA	slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.
98171	p-mCherry-N1-iChloC_CA	slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.
98172	p-mCherry-C1-iChR2++	artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.
98173	p-mCherry-C1-iCoChR++	artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.
98216	AAV2_hSyn_Phobos_Citrine	Blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.
98217	AAV2_hSyn_Aurora_Citrine	Red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.
98218	AAV2_hSyn_Phobos_CA_Citrine	Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.
98219	AAV2_hSyn_Aurora_CA_Citrine	Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.
98220	AAV2_hSyn_iChloC_CA_Citrine	Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.