A CRISPR-Cpf1-Assisted Non-Homologous End Joining Genome Editing System of Mycobacterium smegmatis.
Sun B, Yang J, Yang S, Ye RD, Chen D, Jiang Y
Biotechnol J. 2018 Sep;13(9):e1700588. doi: 10.1002/biot.201700588. Epub 2018 Aug 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 104622 | pSBY1_FnCpf1cg | CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1 |
| 104623 | pSBY2_ligD(FnCpf1) | CRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligD |
| 104624 | pSBY3_ligD | CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD |
| 104625 | pSBY3_ligD-HygR | CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene. |