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Lysosomal metabolomics reveals V-ATPase- and mTOR-dependent regulation of amino acid efflux from lysosomes.

Abu-Remaileh M, Wyant GA, Kim C, Laqtom NN, Abbasi M, Chan SH, Freinkman E, Sabatini DM
Science. 2017 Nov 10;358(6364):807-813. doi: 10.1126/science.aan6298. Epub 2017 Oct 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
102929pLJC5-Tmem192-2xFlagLentiviral expression of a lysosomal tag (Tmem192-Flag)
102930pLJC5-Tmem192-3xHALentiviral expression of a lysosomal tag (Tmem192-HA)
102931pLJC5-LAMP1-RFP-2xFlagLentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag)
102932pLJC5-LAMP1-RFP-3xHALentiviral expression of a lysosomal tag (LAMP1-mRFP1-HA)

Antibodies from Article