Rapid, Selection-Free, High-Efficiency Genome Editing in Protozoan Parasites Using CRISPR-Cas9 Ribonucleoproteins.
Soares Medeiros LC, South L, Peng D, Bustamante JM, Wang W, Bunkofske M, Perumal N, Sanchez-Valdez F, Tarleton RL
MBio. 2017 Nov 7;8(6). pii: e01788-17. doi: 10.1128/mBio.01788-17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 101086 | p6XHis_NLS-SaCas9 | Express SaCas9 in bacteria with a 6xHis tag for purification |