Multiplex recording of cellular events over time on CRISPR biological tape.
Sheth RU, Yim SS, Wu FL, Wang HH
Science. 2017 Dec 15;358(6369):1457-1461. doi: 10.1126/science.aao0958. Epub 2017 Nov 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 104572 | pRec | Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI constitutively. |
| 104573 | pRec-GalS | Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI-GalS chimera constitutively. |
| 104574 | pRec-TreR | Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI-TreR chimera constitutively. |
| 104575 | pRec ΔLacI | Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR constitutively. |
| 104576 | pTrig | Copy number inducible plasmid. Contains mini-F origin for maintenance and P1 lytic replication machinery under Lac promoter for copy increase. |
| 104578 | pTrig-CopA | Copy number inducible plasmid. Contains mini-F origin for maintenance and P1 lytic replication machinery under CopA promoter for copy increase. |