Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors.
Tak YE, Kleinstiver BP, Nunez JK, Hsu JY, Horng JE, Gong J, Weissman JS, Joung JK
Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 104563 | MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA | Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) |
| 104564 | BPK1169: CAG-DmrC-NLS-3xFLAG-P65 | Mammalian expression vector for DmrC fused to p65 activation domain |
| 104565 | MMW948: CAG-DmrC-NLS-3xFLAG-VPR | Mammalian expression vector for DmrC fused to VPR activator |
| 104566 | JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65 | Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to p65 activation domain |
| 104567 | JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR | Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator |
| 104568 | JG674: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X1) | Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domain |
| 104569 | JG676: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X2) | Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to two DmrA domains |
| 104570 | JG693: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X3) | Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to three DmrA domains |
| 104571 | YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4) | Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains |