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Design and Profiling of a Subcellular Targeted Optogenetic cAMP-Dependent Protein Kinase.

O'Banion CP, Priestman MA, Hughes RM, Herring LE, Capuzzi SJ, Lawrence DS
Cell Chem Biol. 2017 Oct 25. pii: S2451-9456(17)30355-0. doi: 10.1016/j.chembiol.2017.09.011. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
104546optoPKAW196R/Y204AExpresses Cry2-mCherry-PKA W196R/Y204A in mammalian cells.
104547optoPKAW196R/F327AExpresses Cry2-mCherry-PKA W196R/F327A in mammalian cells.
104548optoPKAW196RExpresses Cry2-mCherry-PKA W196R in mammalian cells
104550OMM PKA ReporterExpresses Tom20-mTurquoise2-VASP at the outer mitochondrial membrane of mammalian cells.
104551PM PKA ReporterExpresses Lyn-mTurquoise2-VASP at the plasma membrane of mammalian cells

Antibodies from Article