Design and Profiling of a Subcellular Targeted Optogenetic cAMP-Dependent Protein Kinase.

O'Banion CP, Priestman MA, Hughes RM, Herring LE, Capuzzi SJ, Lawrence DS
Cell Chem Biol. 2017 Oct 25. pii: S2451-9456(17)30355-0. doi: 10.1016/j.chembiol.2017.09.011. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
104551	PM PKA Reporter	Expresses Lyn-mTurquoise2-VASP at the plasma membrane of mammalian cells
104550	OMM PKA Reporter	Expresses Tom20-mTurquoise2-VASP at the outer mitochondrial membrane of mammalian cells.
104548	optoPKAW196R	Expresses Cry2-mCherry-PKA W196R in mammalian cells
104547	optoPKAW196R/F327A	Expresses Cry2-mCherry-PKA W196R/F327A in mammalian cells.
104546	optoPKAW196R/Y204A	Expresses Cry2-mCherry-PKA W196R/Y204A in mammalian cells.