CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces cerevisiae.
Verwaal R, Buiting-Wiessenhaan N, Dalhuijsen S, Roubos JA
Yeast. 2017 Sep 8. doi: 10.1002/yea.3278.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 101725 | pCSN061 | Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae. |
| 101748 | pCSN067 | Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae. |
| 101749 | pCSN068 | Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae. |
| 101750 | pRN1120 | Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes. |