Mechanistic View of hnRNPA2 Low-Complexity Domain Structure, Interactions, and Phase Separation Altered by Mutation and Arginine Methylation.
Ryan VH, Dignon GL, Zerze GH, Chabata CV, Silva R, Conicella AE, Amaya J, Burke KA, Mittal J, Fawzi NL
Mol Cell. 2018 Jan 17. pii: S1097-2765(17)30979-6. doi: 10.1016/j.molcel.2017.12.022.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
98657 | hnRNPA2_LC_WT | Expresses 6xHis-tagged hnRNPA2 AA 190-341 |
98658 | hnRNPA2_LC_D290V | Expresses 6xHis-tagged hnRNPA2 AA 190-341 with D290V |
98659 | hnRNPA2_LC_D220V | Expresses 6xHis-tagged hnRNPA2 AA 190-341 with D220V |
98660 | hnRNPA2_LC_D230V | Expresses 6xHis-tagged hnRNPA2 AA 190-341 with D230V |
98661 | MBP-hnRNPA2_LC | Expresses MBP-tagged hnRNPA2 AA 190-341 |
98662 | MBP-hnRNPA2_FL_WT | Expresses MBP-tagged full length hnRNPA2 |
98663 | MBP-hnRNPA2_FL_D290V | Expresses MBP-tagged full length hnRNPA2 with D290V |
98664 | MBP-hnRNPA2_deltaLC | Expresses MBP-tagged hnRNPA2 without the LC domain |
98665 | hnRNPA2_LC_WT_S285C | Expresses 6xHis-tagged hnRNPA2 AA 190-341 with S285C |
98666 | hnRNPA2_LC_D290V_S285C | Expresses 6xHis-tagged hnRNPA2 AA 190-341 with S285C and D290V |
98667 | hnRNPA2_LC_WT_S329C | Expresses 6xHis-tagged hnRNPA2 AA 190-341 with S329C |
98668 | hnRNPA2_LC_D290V_S329C | Expresses 6xHis-tagged hnRNPA2 AA 190-341 with D290V and S329C |
104465 | hnRNPA2_LC_P298L | express His tagged P298L hnRNPA2 LC |
104466 | MBP-hnRNPA2_LC_R191K_R254K | express MBP hnRNPA2 LC with 2 R to K mutations |
104467 | MBP-hnRNPA2_LC_R191K_R201K_R216K_R254K | express MBP hnRNPA2 LC with 4 R to K mutations |
104468 | MBP-hnRNPA2_FL_P298L | express MBP-tagged full length hnRNPA2 P298L |
104469 | hnRNPA2_LC_P298L_S285C | express His tagged P298L S285C hnRNPA2 LC |
104470 | hnRNPA2_LC_P298L_S329C | express His tagged P298L S329C hnRNPA2 LC |