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Mechanistic View of hnRNPA2 Low-Complexity Domain Structure, Interactions, and Phase Separation Altered by Mutation and Arginine Methylation.

Ryan VH, Dignon GL, Zerze GH, Chabata CV, Silva R, Conicella AE, Amaya J, Burke KA, Mittal J, Fawzi NL
Mol Cell. 2018 Jan 17. pii: S1097-2765(17)30979-6. doi: 10.1016/j.molcel.2017.12.022. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
98657hnRNPA2_LC_WTExpresses 6xHis-tagged hnRNPA2 AA 190-341
98658hnRNPA2_LC_D290VExpresses 6xHis-tagged hnRNPA2 AA 190-341 with D290V
98659hnRNPA2_LC_D220VExpresses 6xHis-tagged hnRNPA2 AA 190-341 with D220V
98660hnRNPA2_LC_D230VExpresses 6xHis-tagged hnRNPA2 AA 190-341 with D230V
98661MBP-hnRNPA2_LCExpresses MBP-tagged hnRNPA2 AA 190-341
98662MBP-hnRNPA2_FL_WTExpresses MBP-tagged full length hnRNPA2
98663MBP-hnRNPA2_FL_D290VExpresses MBP-tagged full length hnRNPA2 with D290V
98664MBP-hnRNPA2_deltaLCExpresses MBP-tagged hnRNPA2 without the LC domain
98665hnRNPA2_LC_WT_S285CExpresses 6xHis-tagged hnRNPA2 AA 190-341 with S285C
98666hnRNPA2_LC_D290V_S285CExpresses 6xHis-tagged hnRNPA2 AA 190-341 with S285C and D290V
98667hnRNPA2_LC_WT_S329CExpresses 6xHis-tagged hnRNPA2 AA 190-341 with S329C
98668hnRNPA2_LC_D290V_S329CExpresses 6xHis-tagged hnRNPA2 AA 190-341 with D290V and S329C
104465hnRNPA2_LC_P298Lexpress His tagged P298L hnRNPA2 LC
104466MBP-hnRNPA2_LC_R191K_R254Kexpress MBP hnRNPA2 LC with 2 R to K mutations
104467MBP-hnRNPA2_LC_R191K_R201K_R216K_R254Kexpress MBP hnRNPA2 LC with 4 R to K mutations
104468MBP-hnRNPA2_FL_P298Lexpress MBP-tagged full length hnRNPA2 P298L
104469hnRNPA2_LC_P298L_S285Cexpress His tagged P298L S285C hnRNPA2 LC
104470hnRNPA2_LC_P298L_S329Cexpress His tagged P298L S329C hnRNPA2 LC

Antibodies from Article