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On-demand optogenetic activation of human stem-cell-derived neurons.

Klapper SD, Sauter EJ, Swiersy A, Hyman MAE, Bamann C, Bamberg E, Busskamp V
Sci Rep. 2017 Oct 31;7(1):14450. doi: 10.1038/s41598-017-14827-6. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
104387PB-eDIO-ChR2-EYFPPiggyBac plasmid encoding channelrhodopsin 2 fused to EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.
104388PB-eDIO-EYFPPiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

Antibodies from Article