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Unexpected binding behaviors of bacterial Argonautes in human cells cast doubts on their use as targetable gene regulators.

O'Geen H, Ren C, Coggins NB, Bates SL, Segal DJ
PLoS One. 2018 Mar 27;13(3):e0193818. doi: 10.1371/journal.pone.0193818. eCollection 2018. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
104383pCDNA-hTtAgohuman codon optimized TtAgo; pCDNA3 vector backbone, mammalian expression
104384pCDNA-hNgAgohuman codon optimized NgAgo; pCDNA3 vector backbone, mammalian expression

Antibodies from Article