dCas9-based epigenome editing suggests acquisition of histone methylation is not sufficient for target gene repression.
O'Geen H, Ren C, Nicolet CM, Perez AA, Halmai J, Le VM, Mackay JP, Farnham PJ, Segal DJ
Nucleic Acids Res. 2017 Sep 29;45(17):9901-9916. doi: 10.1093/nar/gkx578.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 100085 | dCas9-FOG1[N+C] | dCas9 fused to two FOG1 peptide [aa 1-45] , one at the N-terminus and one at the C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression |
| 100086 | Ezh2[FL]-dCas9 | Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression |
| 100087 | Ezh2[SET]-dCas9 | Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression |
| 100088 | SUV[SET]-dCas9 | Catalytic domain [SET] of human SUVAR39H1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression |
| 100089 | G9A[SET]-dCas9 | Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression |
| 100090 | DNMT3A-dCas9 | DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression |
| 100091 | dCas9 plasmid | dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression |
| 112195 | KRAB-dCas9 | KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression |