A reference-based protein degradation assay without global translation inhibitors.
Oh JH, Chen SJ, Varshavsky A
J Biol Chem. 2017 Dec 29;292(52):21457-21465. doi: 10.1074/jbc.M117.814236. Epub 2017 Nov 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 104342 | pJO629 | Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability. |