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A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy.

Matela G, Gao P, Guigas G, Eckert AF, Nienhaus K, Nienhaus GU
Chem Commun (Camb). 2017 Jan 10;53(5):979-982. doi: 10.1039/c6cc09081h. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
104308LifeAct-mGarnet2codes for a fusion of fluorescent protein mGarnet2 and lifeact that targets F-actin in live cells
104310mito-mGarnet2codes for a fusion of fluorescent protein mGarnet2 and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito
104312H2B-mGarnet2codes for a fusion of fluorescent protein mGarnet2 and human histon 2B (H2B), makes the H2B visible in live cells
104314mGarnet2-RITAcodes for a fusion of fluorescent protein mGarnet2 and RITA (RBP-J Interacting and tubulin-associated), stains microtubular stucturs in live cells
104316alpha-actinin-mGarnet2codes for a fusion of fluorescent protein mGarnet2 and human alpha-actin, visualizes human alpha-actinin
104318mGarnet2-alpha-tubulincodes for a fusion of fluorescent protein mGarnet2 and alpha-tubulin, visualizes alpha-tubulin

Antibodies from Article