Regulation of DNA repair pathway choice in S and G2 phases by the NHEJ inhibitor CYREN.
Arnoult N, Correia A, Ma J, Merlo A, Garcia-Gomez S, Maric M, Tognetti M, Benner CW, Boulton SJ, Saghatelian A, Karlseder J
Nature. 2017 Sep 20;549(7673):548-552. doi: 10.1038/nature24023.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 98895 | pLCN DSB Repair Reporter (DRR) | DSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ. |
| 98896 | pCAGGS DRR mCherry Donor EF1a BFP | Homologous recombination donor for pDRR. Expresses mCherry after repait by HR. |
| 98970 | pCAGGS Donor mClover-LMNA | Donor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination Reporter |
| 98971 | pUC CBA-SpCas9.EF1a-BFP.sgLMNA | Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP. |
| 98972 | pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1 | Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP. |
| 98973 | pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2 | Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP. |
| 98974 | pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3 | Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP. |
| 98975 | pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4 | Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP. |
| 98976 | pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1 | Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP. |