Real-time observation of light-controlled transcription in living cells.
Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. Epub 2017 Nov 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 103797 | pCIBN-tagBFP-TetR | BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light |
| 103798 | pCIBN-EYFP-TetR | BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light |
| 103799 | pCIBN-tagBFP-hLaminB1 | BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light |
| 103800 | pCIBN-EYFP-hLaminB1 | BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light |
| 103801 | pCIBN-tagBFP-hTRF1 | BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light |
| 103802 | pCIBN-EYFP-hTRF1 | BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light |
| 103803 | pCIBN-tagBFP-hTRF2 | BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light |
| 103804 | pCIBN-EYFP-hTRF2 | BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light |
| 103805 | pCIBN-tagBFP-hNCL | BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light |
| 103806 | pCIBN-EYFP-hNCL | BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light |
| 103807 | pCIBN-tagBFP-hPMLIII | BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light |
| 103808 | pCIBN-EYFP-hPMLIII | BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light |
| 103809 | pCIBN-TetR-tagRFP-T | 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment |
| 103810 | pCIBN-LacI-tagRFP-T | 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment |
| 103811 | pCIBN-hTRF1-tagRFP-T | BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment |
| 103812 | pCIBN-hTRF2-tagRFP-T | BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment |
| 103813 | pCIBN-TetR | BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light |
| 103814 | pCIBN-LacI | BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light |
| 103815 | pCIBN-EYFP | Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light |
| 103816 | pCIBN-mCherry | Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light |
| 103817 | pPHR-EYFP | Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination |
| 103818 | pPHR-iRFP713 | Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination |
| 103819 | pPHR-mCherry-NLS | Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination |
| 103820 | pPHR-EYFP-NLS | Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination |
| 103821 | pPHR-mCherry-NLS-VP16 | Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination |
| 103822 | pPHR-EYFP-NLS-VP16 | Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination |
| 103823 | pPHR-iRFP713-NLS-VP16 | Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination |
| 103824 | pPHR-mCherry-hPMLIII | Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination |
| 103825 | pPHR-EYFP-hPMLIII | Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination |
| 103826 | pPHR-EYFP-hGCN5 | Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination |
| 103827 | pPHR-EYFP-hGCN5-NLS | Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination |
| 103828 | pPHR-EYFP-hGCN5mut | Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination |
| 103829 | pPHR-EYFP-hHP1β | Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination |
| 103830 | pPHR-mCherry-hHP1β | Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination |
| 103831 | ptagRFP-MS2coatProtein | Live cell reader for MS2 loop RNA production |
| 103832 | pmCherry-MS2coatProtein | Live cell reader for MS2 loop RNA production |
| 103833 | pmKate2-MS2coatProtein | Live cell reader for MS2 loop RNA production |
| 103834 | pEGFP-TetR-NLS-VP16 | Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays |
| 103835 | ptagBFP-TetR-NLS-VP16 | Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays |
| 103836 | pEGFP-LacI-NLS-VP16 | Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays |
| 103837 | ptagBFP-LacI-NLS-VP16 | Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays |
| 103838 | pNLS-TetR-GFP | Flourescent marker for tetO arrays |
| 103839 | ptagBFP-LacI | Flourescent marker for lacO arrays |
| 103840 | pCFP-LacI-NLS | Flourescent marker for lacO arrays |