High-throughput construction of multiple cas9 gene variants via assembly of high-depth tiled and sequence-verified oligonucleotides.
Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, Lee JH, Kwon S, Bang D
Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 103070 | pUC19-A0LWB3 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103071 | pCAG-B2UP49_GFP | Cas9 coding gene template with optimized sequence for human codon usage, also expresses EGFP |
| 103072 | pUC19-B9M9X8 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103074 | pUC19-D3NT09 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103075 | pUC19-E3CY56 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103076 | pUC19-E6KTN8 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103077 | pUC19-F4GDP9 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103078 | pUC19-J9E534 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103079 | pUC19-K2FTY8 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103080 | pUC19-A1IQ68 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103081 | pUC19-A5EIM8 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103082 | pUC19-A7HP89 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103083 | pUC19-A8LN05 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103084 | pUC19-A8REH9 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103085 | pUC19-B1UZL4 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103086 | pUC19-B2KB46 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103087 | pUC19-B5JU35 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103088 | pUC19-B5XLC1 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103089 | pUC19-B8I085 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103090 | pUC19-C4ZA16 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103091 | pUC19-C7G697 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103092 | pUC19-C7TEQ6 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103093 | pUC19-D1VXP4 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103095 | pUC19-D3UFL8 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103096 | pUC19-D4J3S7 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103097 | pUC19-D6GRK4 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103098 | pUC19-E0XXB7 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103099 | pUC19-E3ELL7 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103100 | pUC19-E3HCA8 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103101 | pUC19-E6I4Q5 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103102 | pUC19-E6MBW6 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103103 | pUC19-E6WZS9 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103104 | pUC19-E7H313 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103105 | pUC19-E7MR72 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103106 | pUC19-E9S7M7 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103107 | pUC19-F3QMY7 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103108 | pUC19-F3UXG6 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103110 | pUC19-G2ZYP2 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103111 | pUC19-G9QLF2 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103112 | pUC19-G9WGU4 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103113 | pUC19-H1DEI0 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103114 | pUC19-I0X7Z6 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103115 | pUC19-I0ZZR5 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103116 | pUC19-J5TNY4 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103117 | pUC19-J6LE60 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103118 | pUC19-K0XCK7 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103119 | pUC19-K1LM04 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103120 | pUC19-Q0P897 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103121 | pUC19-Q1QGC9 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103122 | pUC19-Q2RX87 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103123 | pUC19-Q03JI6 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103124 | pUC19-Q03LF7 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103125 | pUC19-Q4A5I2 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103126 | pUC19-Q5L8F8 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103127 | pUC19-Q5X8T6 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103128 | pUC19-Q6KIQ7 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103129 | pUC19-Q8DTE3 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103130 | pUC19-Q73QW6 | Cas9 coding gene template with optimized sequence for human codon usage |
| 103131 | pUC19-Q927P4 | Cas9 coding gene template with optimized sequence for human codon usage |