Differentiation of Human Pluripotent Stem Cells into Functional Lung Alveolar Epithelial Cells.
Jacob A, Morley M, Hawkins F, McCauley KB, Jean JC, Heins H, Na CL, Weaver TE, Vedaie M, Hurley K, Hinds A, Russo SJ, Kook S, Zacharias W, Ochs M, Traber K, Quinton LJ, Crane A, Davis BR, White FV, Wambach J, Whitsett JA, Cole FS, Morrisey EE, Guttentag SH, Beers MF, Kotton DN
Cell Stem Cell. 2017 Oct 5;21(4):472-488.e10. doi: 10.1016/j.stem.2017.08.014. Epub 2017 Sep 28.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 126694 | EF1a-TALEN_NN (SPC left) | left TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site |
| 126695 | EF1a-TALEN_HD (SPC right) | right TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site |
| 126696 | p1303 DV-SFTPC-tdTomato | donor vector for targeting a tdTomato reporter to the human SFTPC locus at the endogenous ATG start site |
| 126700 | pHAGE2-EF1aL-Cre-IRES-NeoR-W | lentiviral or plasmid based co-expression of Cre recombinase and a neomycin resistance cassette |