Model-guided optogenetic study of PKA signaling in budding yeast.
Stewart-Ornstein J, Chen S, Bhatnagar R, Weissman JS, El-Samad H
Mol Biol Cell. 2017 Jan 1;28(1):221-227. doi: 10.1091/mbc.E16-06-0354. Epub 2016 Nov 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 103017 | pHES609 [prNOp7-PBAC] | A S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA. |
| 103844 | pHES262 [prADH1-Msn2-mCherry, pnh604] | Yeast Expression Plasmid |
| 103846 | pHES553 [prGal1-RAS2(KAN) pnh604] | Yeast Expression Plasmid |