RNA targeting with CRISPR-Cas13.

Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, Voytas DF, Ting AY, Zhang F
Nature. 2017 Oct 4. doi: 10.1038/nature24049. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
91902	pC014 - LwCas13a-msfGFP	Expresses active LwCas13a for mammalian RNA targeting
91905	pC015 - dLwCas13a-NF	Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging
91906	pC016 - LwCas13a guide expression backbone with U6 promoter	Backbone for cloning LwCas13a guides under U6 promoter
91907	pC017 - LwCas13a guide expression backbone with tRNAval promoter	Backbone for cloning LwCas13a guides under tRNAval promoter
91908	pC018 - LshCas13a from Leptotrichia shahii	Expresses LshCas13a for bacterial expression and contains backbone for spacer cloning
91909	pC019 - LwCas13a from Leptotrichia wadei	Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning
91910	pC020 - LseCas13a from Listeria seeligeri	Expresses LseCas13a for bacterial expression and contains backbone for spacer cloning
91911	pC021 - LbmCas13a from Lachnospiraceae bacterium MA2020	Expresses LbmCas13a for bacterial expression and contains backbone for spacer cloning
91912	pC022 - LbnCas13a from Lachnospiraceae bacterium NK4A179	Expresses LbnCas13a for bacterial expression and contains backbone for spacer cloning
91913	pC023 - CaCas13a from [Clostridium] aminophilum DSM 10710	Expresses CaCas13a for bacterial expression and contains backbone for spacer cloning
91914	pC024 - CgCas13a from Carnobacterium gallinarum DSM 4847	Expresses CgCas13a for bacterial expression and contains backbone for spacer cloning
91915	pC025 - Cg2Cas13a from Carnobacterium gallinarum DSM 4847	Expresses Cg2Cas13a for bacterial expression and contains backbone for spacer cloning
91916	pC026 - PpCas13a from Paludibacter propionicigenes WB4	Expresses PpCas13a for bacterial expression and contains backbone for spacer cloning
91917	pC027 - LweCas13a from Listeria weihenstephanensis FSL R9-0317	Expresses LweCas13a for bacterial expression and contains backbone for spacer cloning
91918	pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635	Expresses LbfCas13a for bacterial expression and contains backbone for spacer cloning
91919	pC029 - Lw2Cas13a from Leptotrichia wadei F0279	Expresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloning
91920	pC030 - RcsCas13a from Rhodobacter capsulatus SB 1003	Expresses RcsCas13a for bacterial expression and contains backbone for spacer cloning
91921	pC031 - RcrCas13a from Rhodobacter capsulatus R121	Expresses RcrCas13a for bacterial expression and contains backbone for spacer cloning
91922	pC032 - RcdCas13a from Rhodobacter capsulatus DE442	Expresses RcdCas13a for bacterial expression and contains backbone for spacer cloning
91924	pC034 - LwCas13a-msfGFP-2A-Blast	Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker
91925	pC035 - dLwCas13a-msfGFP	Expresses catalytically inactive LwCas13a for mammalian RNA targeting
105815	pC0056 - LwCas13a-msfGFP-NES	Expresses active LwaCas13a-NES for mammalian RNA targeting
107691	pJJB296	35SPromoter-LwaCas13a-HSPTerminator
107694	pJJB302	OsActin Promoter-LwaCas13a-HSP Terminator
107699	pJJB308	AtU6 Promoter-LwaCas13a-gRNA Scaffold