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A multiplexable TALE-based binary expression system for in vivo cellular interaction studies.

Toegel M, Azzam G, Lee EY, Knapp DJHF, Tan Y, Fa M, Fulga TA
Nat Commun. 2017 Nov 21;8(1):1663. doi: 10.1038/s41467-017-01592-3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
104605pJC-20xUAS-TALE-VP64-P10Control plasmid (no TALE array) with P10 terminator
104606pJC-20xUAS-TALE1-VP64-P10Expresses TALE1 under UAS control
104607pJC-20xUAS-TALE3-VP64-P10Expresses TALE3 under UAS control
104608pJC-20xUAS-TALE4-VP64-P10Expresses TALE4 under UAS control
104609pJC-elav1.8kb-TALE1-VP64-P10Expresses TALE1 under the control of a 1.8kb elav enhancer element
104610pJC-ase0.8kb-TALE3-VP64-P10Expresses TALE3 under the control of a 0.8kb ase enhancer element
104611pJC-mhc2.4kb-TALE3-VP64-P10Expresses TALE3 under the control of a 2.4kb mhc enhancer element
104612pJC-repo1.9kb-TALE4-VP64-P10Expresses TALE4 under the control of a 1.9kb repo enhancer element
104613pJFRC81-3xVAS1-IVS-Syn21-GFP-P10Expresses GFP upon binding of TALE1 to VAS1
104614pJFRC81-3xVAS3-IVS-Syn21-GFP-P10Expresses GFP upon binding of TALE3 to VAS3
104615pJFRC81-3xVAS4-IVS-Syn21-GFP-P10Expresses GFP upon binding of TALE4 to VAS4
104616pJFRC81-3xVAS1-Syn21-Citrine-HA-P10Expresses HA tagged Citrine upon binding of TALE1 to VAS1
104617pJFRC81-3xVAS3-Syn21-V5-mCherry-P10Expresses V5 tagged mCherry upon binding of TALE3 to VAS3
104618pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10Expresses FLAG tagged Cerulean upon binding of TALE4 to VAS4
104619pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10Expresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4

Antibodies from Article