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A Single-Chain Photoswitchable CRISPR-Cas9 Architecture for Light-Inducible Gene Editing and Transcription.

Zhou XX, Zou X, Chung HK, Gao Y, Liu Y, Qi LS, Lin MZ
ACS Chem Biol. 2017 Sep 29. doi: 10.1021/acschembio.7b00603. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
102851U6-hGRIN2B-CAG-ps-SpCas9A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing
102853U6-hGRIN2B-CAG-ps-SaCas9A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing
102854U6-spTRE3G-CMV-mTagBFP2A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP
102855VPR-ps-dSpCas9A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains

Antibodies from Article