Donna Webb Lab: Webb Lab plasmids
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 102783 | APPL1-N308D-GFP | Point mutation in APPL1 that diminishes its interaction with Rab5 |
| 102784 | APPL1-N308D-mCherry | Point mutation in APPL1 that diminishes its interaction with Rab5 |
| 102785 | Rab5-L38R-mCherry | Point mutation in Rab5 that reestablishes interaction with APPL1 when co-expressed with APPL1-N308D |
| 102998 | pET22B_PcrA_2Bchimera | Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA |
| 102999 | pET22B_SA_PcrA | The plasmid is used to express Staphylococcus aureus PcrA, a DNA helicase, with N-terminal His-tag and Gly-Gly linker |