Genome-wide CRISPR Screens in T Helper Cells Reveal Pervasive Crosstalk between Activation and Differentiation.
Henriksson J, Chen X, Gomes T, Ullah U, Meyer KB, Miragaia R, Duddy G, Pramanik J, Yusa K, Lahesmaa R, Teichmann SA
Cell. 2019 Jan 7. pii: S0092-8674(18)31569-1. doi: 10.1016/j.cell.2018.11.044.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 102796 | pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP | Retrovirus for delivery of one sgRNA (empty, bbsI) |
| 102797 | pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP | Retrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroR |
| 102798 | pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP | Retrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroR |
| 104861 | pMSCV-U6gRNA(lib)-PGKpuroT2ABFP library | |
| 117263 | MSCV-Bhlhe40-3xFLAG-IRES-BFP | Retroviral overexpression of Bhlhe40 with a 3xFLAG |
| 117265 | MSCV-Scara3-IRES-BFP | Retroviral overexpression of Scara3 |
| 117266 | MSCV-Ccdc134-IRES-BFP | Retroviral overexpression of Ccdc134 |
| 117268 | MSCV-Lrrc40-trunc-IRES-BFP | Retroviral overexpression of Lrrc40 - truncated version with seemingly same DE genes in Th2 as MSCV-Lrrc40-IRES-BFP |