Generation of a SOX2 reporter human induced pluripotent stem cell line using CRISPR/SaCas9.
Balboa D, Weltner J, Novik Y, Eurola S, Wartiovaara K, Otonkoski T
Stem Cell Res. 2017 Jul;22:16-19. doi: 10.1016/j.scr.2017.05.005. Epub 2017 May 17.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
89991 | pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro | Donor template for generation of SOX2-ntdTomato reporter cell lines |
89996 | CAG-SaCas9-WPRE | Expresses high levels of WT SaCas9 for genome editing |