Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors.
Baeumler TA, Ahmed AA, Fulga TA
Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 101101 | pTMt_NES_TCS(Q’G)_HA-dCas9(N)_P2A-Puro-WPRE | Encodes dCas9(N) fused to transmembrane tether |
| 101102 | pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1 | Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether |
| 101103 | pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1 | Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1 |
| 101104 | pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE | Encodes dCas9(N) fused to VEGFR2 |
| 101105 | pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C) | Encodes dCas9(C) (without VP64) fused to VEGFR1 |
| 101106 | pVEGFR2_TEV(N)_NES_TCS(Q'L)_FKBP_HA-dCas9(N)_P2A_Puro-WPRE | Encodes FKBP-dCas9(N) fused to VEGFR2 |
| 101107 | pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1 | Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2 |
| 101108 | pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE | Encodes dCas9(N) fused to BDKRB2 |
| 101109 | pBDKRB2_TCS(Q’L)_HA-dCas9(N)_DHFR | Encodes dCas9(N)-DHFR fused to BDKRB2 |
| 101110 | pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)_T2A_DHFR-PCP-VP64 | Encodes dCas9(C) with DHFR-PP7-VP64 fused to BDKRB2 |
| 101111 | pAVPR2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1 | Encodes dCas9(C)-VP64 with SAM components fused to AVPR2 |
| 101112 | pAVPR2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE | Encodes dCas9(N) fused to AVPR2 |
| 101113 | pCXCR4_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1 | Encodes dCas9(C)-VP64 with SAM components fused to CXCR4 |
| 101114 | pCXCR4_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE | Encodes dCas9(N) fused to CXCR4 |
| 101115 | pLPAR1_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1 | Encodes dCas9(C)-VP64 with SAM components fused to LPAR1 |
| 101116 | pLPAR1_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE | Encodes dCas9(N) fused to LPAR1 |