Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2.
Mastop M, Bindels DS, Shaner NC, Postma M, Gadella TWJ Jr, Goedhart J
Sci Rep. 2017 Sep 20;7(1):11999. doi: 10.1038/s41598-017-12212-x.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 98835 | Gq-sensor[mTq2-sREACh] | A FRET sensor for Gq activation, encoded on a single plasmid, for FLIM |
| 98837 | mKOkappa-2A-mTurquoise2 | Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET |
| 98838 | mKO2-2A-mTurquoise2 | Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET |
| 98839 | mScarlet-I-mTurquoise2 | Produces a fusion between mTurquoise2 and mScarletI. It can be used as a positive control for FRET |
| 98840 | sReach-mTurquoise2 | Produces a fusion between mTurquoise2 and sReach. It can be used as a positive control for FRET (using FLIM) |
| 98884 | Gq-sensor[mTq2-mNG] | A FRET sensor for Gq activation, encoded on a single plasmid. |
| 98885 | mNeonGreen-2A-mTurquoise2 | Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET |
| 98886 | mNeonGreen-mTurquoise2 | Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET |