CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing.
Moreno-Mateos MA, Fernandez JP, Rouet R, Vejnar CE, Lane MA, Mis E, Khokha MK, Doudna JA, Giraldez AJ
Nat Commun. 2017 Dec 8;8(1):2024. doi: 10.1038/s41467-017-01836-2.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 102565 | AsCpf1-2NLS | bacterial expression of AsCpf1-2NLS |
| 102566 | LbCpf1-2NLS | bacterial expression of LbCpf1-2NLS |
| 107185 | pT3TS-zAsCpf1 | plasmid to carry out IVT of AsCpf1 (zebrafish codon optimized) |
| 107187 | pSP64T-hLbCpf1 | plasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988) |