One-Step piggyBac Transposon-Based CRISPR/Cas9 Activation of Multiple Genes.
Li S, Zhang A, Xue H, Li D, Liu Y
Mol Ther Nucleic Acids. 2017 Sep 15;8:64-76. doi: 10.1016/j.omtn.2017.06.007. Epub 2017 Jun 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 102559 | PB-SAM | Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. |
| 102560 | PB-sgRNA(MS2) | Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences. |
| 102563 | PB-SAM-Dest | Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes. |