Generation of an OCT4 reporter human induced pluripotent stem cell line using CRISPR/SpCas9.
Balboa D, Weltner J, Novik Y, Eurola S, Wartiovaara K, Otonkoski T
Stem Cell Res. 2017 Aug;23:105-108. doi: 10.1016/j.scr.2017.07.006. Epub 2017 Jul 11.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
89992 | pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro | Donor template for generation of OCT4-T2A-nEmGFP reporter cell lines |
89995 | CAG-Cas9 | Expresses high levels of WT Cas9 for genome editing |