Construction of Incoherent Feedforward Loop Circuits in a Cell-Free System and in Cells.
Guo S, Murray RM
ACS Synth Biol. 2019 Mar 15;8(3):606-610. doi: 10.1021/acssynbio.8b00493. Epub 2019 Mar 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 102449 | pSG13-pBAD-TetRssrA | Expresses TetR protein with ssrA degradation tag. The expression is controlled by a pBAD promoter. |
| 102450 | pSG15-pBAD-tetO-deGFP | Expresses deGFP fluorescent protein in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter. |
| 102451 | pSG22-pBAD-TetR | Expresses TetR protein in E. coli. The expression is controlled by a pBAD promoter. |
| 102452 | pSG25-pBAD-tetO-deGFPssrA | Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter. |
| 102453 | pSG28-J23151-AraC | Expresses AraC protein in E. coli. The expression is controlled by a constitutive promoter. |
| 102454 | pSG81-pLas-tetO-deGFPssrA | Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter. |
| 102455 | pSG84-pLas-TetRssrA | Expresses TetR protein with ssrA degradation tag. The expression is controlled by a pLas promoter. |
| 102456 | pSG86-J23151-LasR | Expresses LasR protein in E. coli. The expression is controlled by a constitutive promoter. |