CRISPR Guide RNA Cloning for Mammalian Systems.
Nageshwaran S, Chavez A, Cher Yeo N, Guo X, Lance-Byrne A, Tung A, Collins JJ, Church GM
J Vis Exp. 2018 Oct 2;(140). doi: 10.3791/57998.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 102440 | pSN007 | For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046) |