Lineage-specific functions of TET1 in the postimplantation mouse embryo.
Khoueiry R, Sohni A, Thienpont B, Luo X, Velde JV, Bartoccetti M, Boeckx B, Zwijsen A, Rao A, Lambrechts D, Koh KP
Nat Genet. 2017 Jul;49(7):1061-1072. doi: 10.1038/ng.3868. Epub 2017 May 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 102421 | pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV | Inducible expression of siRNA resistant mouse Tet1-201 (Ensembl transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in piggyBac (PB) transposon vector |
| 102422 | pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV | Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector |
| 102423 | pPB-CAG-rtTA-IRES-Hygro | Mammalian expression vector for tetracycline-transactivator (rtTA), IRES-Hygro |