Tracing Information Flow from Erk to Target Gene Induction Reveals Mechanisms of Dynamic and Combinatorial Control.
Wilson MZ, Ravindran PT, Lim WA, Toettcher JE
Mol Cell. 2017 Sep 7;67(5):757-769.e5. doi: 10.1016/j.molcel.2017.07.016. Epub 2017 Aug 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 102350 | pHR SFFVp BFP-Erk D319N | Lentiviral expressing translational fusion between blue fluorescent protein and Erk2 bearing the D319N mutation |
| 102351 | pHR SFFVp MCP-mCherry IRES h2b-diRFP | Lentiral expression vector bearing the MS2 Coat Protein (MCP) fused to mCherry as well as the nuclear marker histone 2b fused to two copies of irFP. Also bears the hygromycin selection marker. |
| 131775 | pAAV Fos YFP MS2 | Fos upstream msfYFP 24xMS2 Fos downstream for CRISPR tagging |
| 131776 | pAAV Btg2 YFP MS2 | Btg2 upstream msfYFP 24xMS2 Btg2 downstream for CRISPR tagging |
| 131777 | pAAV RhoB YFP MS2 | RhoB upstream msfYFP 24xMS2 RhoB downstream for CRISPR tagging |