Targeted DNA methylation in human cells using engineered dCas9-methyltransferases.
Xiong T, Meister GE, Workman RE, Kato NC, Spellberg MJ, Turker F, Timp W, Ostermeier M, Novina CD
Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.1038/s41598-017-06757-0.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89930 | pCMV sMTase (eGFP) | Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter. |
| 89931 | pCMV dCas9-MC (eGFP) | Expresses the dCas9-MC fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter. |
| 89932 | pCMV MN (eGFP) | Expresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter. |