Switching of metabolic programs in response to light availability is an essential function of the cyanobacterial circadian output pathway.
Puszynska AM, O'Shea EK
Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 101826 | kaiBC- | deletes kaiBC locus |
| 101828 | P0050::kaiBC | drives expression of kaiBC from an rpaA-independent promoter |
| 101829 | rpaA_NS1 | drives expression of rpaA from the NS1 site in the S. elongatus PCC7942 genome |
| 101830 | P0050::rpaA | drives expression of rpaA from an rpaA-independent promoter |
| 101831 | P0050::rpaAD53A | drives expression of rpaA D53A variant from an rpaA-independent promoter |
| 101836 | P0050::kaiBC S431E T432E NS1 | drives expression of kaiBC S431E T432E variant from an rpaA-independent promoter in NS1 locus |
| 101837 | P0050::kaiBC S431A T432A NS1 | drives expression of kaiBC S431A T432A variant from an rpaA-independent promoter in NS1 locus |
| 101838 | glgP-gap1- | deletes glgP_gap1 locus |
| 101839 | zwf-fbp-opcA- | deletes zwf_fbp_opcA locus |
| 101840 | gnd- | deletes gnd locus |
| 101841 | glgA- | deletes glgA locus |
| 101842 | glgC- | deletes glgC locus |
| 101843 | Ptrc::galP_NS1 | drives expression of galP transporter from NS1 locus |
| 101844 | Ptrc::gnd | drives expression of gnd using Ptrc promoter |
| 101845 | Ptrc::glgP_gap1 | Replaces native promoter of glgP_gap1 with Ptrc promoter |
| 101846 | Ptrc::zwf_fbp_opcA | Replaces native promoter of zwf_fbp_opcA with Ptrc promoter |
| 101847 | rpaA- | deletes rpaA |
| 102321 | P0050::rpaA (KmR) | drives expression of rpaA from an rpaA-independent promoter (kanamycin resistance casette) |
| 105229 | Ptrc::RpaA D53A | inducible expression of unphosphorylatable variant of RpaA (D53A) |