Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis.
Pontes-Quero S, Heredia L, Casquero-Garcia V, Fernandez-Chacon M, Luo W, Hermoso A, Bansal M, Garcia-Gonzalez I, Sanchez-Munoz MS, Perea JR, Galiana-Simal A, Rodriguez-Arabaolaza I, Del Olmo-Cabrera S, Rocha SF, Criado-Rodriguez LM, Giovinazzo G, Benedito R
Cell. 2017 Aug 10;170(4):800-814.e18. doi: 10.1016/j.cell.2017.07.031.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 99613 | LoxP1-Mb2YFP-2A (SO244) | To clone gene of interest downstream of LoxP1-Mb2YFP-2A cassette |
| 99614 | LoxP2-Mb2Tomato-2A (SO240) | To clone gene of interest downstream of LoxP2-Mb2Tomato-2A cassette |
| 99615 | LoxP3-Mb2-HA-mTFP1-2A (SO157) | To clone gene of interest downstream of LoxP3-Mb2-HA-mTFP1-2A |
| 99616 | LoxP1-His-H2B-Cherry-2A (SO290) | To clone gene of interest downstream of LoxP1-His-H2B-Cherry-2A cassette |
| 99617 | LoxP2-H2B-EGFP-V5-2A (SO107) | To clone gene of interest downstream of LoxP2-H2B-EGFP-V5-2A |
| 99618 | LoxP3-HA-H2B-Cerulean-2A (SO104) | To clone gene of interest downstream of LoxP3-HA-H2B-Cerulean-2A |
| 99619 | FRT1-Mb2YFP-2A (IG153) | To clone gene of interest downstream of FRT1-Mb2YFP-2A cassette |
| 99620 | FRT2-Mb2Tomato-2A (IG154) | To clone gene of interest downstream of FRT2-Mb2Tomato-2A cassette |
| 99621 | FRT3-Mb2-HA-mTfp1-2A (IG155) | To clone gene of interest downstream of FRT3-Mb2-HA-mTfp1-2A |
| 99622 | FRT1-His-H2B-Cherry-2A (IG156) | To clone gene of interest downstream of FRT1-His-H2B-Cherry-2A cassette |
| 99623 | FRT2-H2B-EGFP-V5-2A (IG157) | To clone gene of interest downstream of FRT2-H2B-EGFP-V5-2A cassette |
| 99624 | FRT3-HA-H2B-Cerulean-2A (IG158)) | To clone gene of interest downstream of FRT3-HA-H2B-Cerulean-2A cassette |
| 99625 | Triple ORF LoxP ifgMosaic Donor (LH208) | To clone the 3 ORFs for Cre dependent generation of ifgMosaics |
| 99626 | Triple ORF FRT ifgMosaic Donor (IG135) | To clone the 3 ORFs for FlpO dependent generation of ifgMosaics |
| 99627 | pRosa26-4xInsulator (JP6) | IfgMosaic acceptor vector for Classical or Cas9 Gene targeting of the Rosa26 locus |
| 99628 | px330 Rosa26 AscI LH291Gu1 (LH500) | Plasmid used to target the pre-modified Rosa26 allele with Cas9 |
| 99629 | px330 Rosa26 Gu1 (LH416) | Plasmid used to target the wildtype Rosa26 allele with Cas9 |
| 99630 | Tre-Tight ifgMosaic Donor vector (LH121) | To clone the 3 ORFs for Tet system (rtTA + Dox) dependent generation of ifgMosaics |
| 99631 | 4xnr UAS ifgMosaic Donor vector (IG230) | To clone the 3 ORFs for Gal4 dependent generation of ifgMosaics |
| 99632 | pRosa26-Acceptor (AG103) | To insert the Tre-Tight or UAS ifgMosaic Donor vectors |
| 99748 | iMb-Control-Mosaic (IR98.10) | Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different fluorescent and marker proteins localized to the membrane |
| 99749 | iMb-Notch-Mosaic (IR99.40) | Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins and Notch signalling genes |
| 99750 | iMb2-Control-Mosaic (SO273) | Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins |
| 99751 | iChr2-Control-Mosaic (SO274) | Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different chromatin localized fluorescent proteins |
| 99752 | iChr2-Notch Mosaic (SO250) | Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different chromatin localized fluorescent proteins and Notch signalling genes |