Tuning dCas9's ability to block transcription enables robust, noiseless knockdown of bacterial genes.
Vigouroux A, Oldewurtel E, Cui L, Bikard D, van Teeffelen S
Mol Syst Biol. 2018 Mar 8;14(3):e7899.
(Link opens in a new window)
PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 101803 | pCRRNA-G0-R0 | Repress mCherry and GFP independently, with different strengths |
| 101804 | pCRRNA-G10-R0 | Repress mCherry and GFP independently, with different strengths |
| 101805 | pCRRNA-G11-R0 | Repress mCherry and GFP independently, with different strengths |
| 101806 | pCRRNA-G14-R0 | Repress mCherry and GFP independently, with different strengths |
| 101807 | pCRRNA-G20-R0 | Repress mCherry and GFP independently, with different strengths |
| 101808 | pCRRNA-G0-R11 | Repress mCherry and GFP independently, with different strengths |
| 101809 | pCRRNA-G10-R11 | Repress mCherry and GFP independently, with different strengths |
| 101810 | pCRRNA-G11-R11 | Repress mCherry and GFP independently, with different strengths |
| 101811 | pCRRNA-G14-R11 | Repress mCherry and GFP independently, with different strengths |
| 101812 | pCRRNA-G20-R11 | Repress mCherry and GFP independently, with different strengths |
| 101813 | pCRRNA-G0-R18 | Repress mCherry and GFP independently, with different strengths |
| 101814 | pCRRNA-G10-R18 | Repress mCherry and GFP independently, with different strengths |
| 101815 | pCRRNA-G11-R18 | Repress mCherry and GFP independently, with different strengths |
| 101816 | pCRRNA-G14-R18 | Repress mCherry and GFP independently, with different strengths |
| 101817 | pCRRNA-G20-R18 | Repress mCherry and GFP independently, with different strengths |
| 101818 | pCRRNA-G0-R20 | Repress mCherry and GFP independently, with different strengths |
| 101819 | pCRRNA-G10-R20 | Repress mCherry and GFP independently, with different strengths |
| 101820 | pCRRNA-G11-R20 | Repress mCherry and GFP independently, with different strengths |
| 101821 | pCRRNA-G14-R20 | Repress mCherry and GFP independently, with different strengths |
| 101822 | pCRRNA-G20-R20 | Repress mCherry and GFP independently, with different strengths |