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DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.

Samwer M, Schneider MWG, Hoefler R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW
Cell. 2017 Aug 24;170(5):956-972.e23. doi: 10.1016/j.cell.2017.07.038. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
101772EGFP-BAFExpresses EGFP tagged BAF in human cells
101773EGFP-P2A_BAFExpresses BAF in human cells (with EGFP produced from the same transcript as expression control)
101774EGFP-P2A_BAF_G47EExpresses mutant BAF (G47E) in human cells (with EGFP produced from the same transcript as expression control)
101775EGFP-P2A_BAF_L58RExpresses mutant BAF (L58R) in human cells (with EGFP produced from the same transcript as expression control)
101776EGFP-BAF_L58RExpresses EGFP tagged mutant BAF (L58R) in human cells
101777H14_MBP_SUMO-Cys-linker-BAFExpresses His-MBP-tagged BAF in E.coli (SUMO-cleavable)
101778H14-MBP-SUMO-FLAG-LEMExpresses His-MBP-tagged mutant LEM-domain (FLAG-tagged) in E.coli (SUMO-cleavable)
101779H14-MBP-SUMO-Cys-Linker-BAF_G47EExpresses His-MBP-tagged mutant BAF (G47E) in E.coli (SUMO-cleavable)
101780H14-MBP-SUMO-Cys-Linker-BAF_L58RExpresses His-MBP-tagged mutant BAF (L58R) in E.coli (SUMO-cleavable)

Antibodies from Article