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Addgene

In Situ Capture of Chromatin Interactions by Biotinylated dCas9.

Liu X, Zhang Y, Chen Y, Li M, Zhou F, Li K, Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu J
Cell. 2017 Aug 24;170(5):1028-1043.e19. doi: 10.1016/j.cell.2017.08.003. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
100547pEF1a-FB-dCas9-puroExpresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein
100548pEF1a-BirA-V5-neoExpresses C-terminal V5-tagged BirA biotin ligase
100549pSLQ1651-sgRNA(F+E)-sgGal4sgGal4-expressing vector modified from Addgene plasmid #51024

Antibodies from Article